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Trinity

Trinity fully reconstructs a large fraction of transcripts, including alternatively spliced isoforms, transcripts from recently duplicated genes and transcriptomes from Illumina RNA-seq data.

Trinity is available as a module on Apocrita.

Usage

To run the default installed version of Trinity, simply load the trinity module:

module load trinity

For usage documentation, run Trinity -h.

Example job

Serial job

Here is an example job running on 2 cores and 10GB of memory:

#!/bin/bash
#$ -cwd
#$ -j y
#$ -pe smp 2
#$ -l h_rt=1:0:0
#$ -l h_vmem=5G

module load trinity

Trinity --seqType fq \
        --max_memory 10G \
        --left reads.left.fq.gz \
        --right reads.right.fq.gz \
        --CPU ${NSLOTS}

References